ERN1 Knockout cell line (A549)

location： Home > Products > KO Cell Lines > ERN1 Knockout cell line (A549)

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

ERN1 Knockout cell line (A549) Order

Catalog#

YKO-H818

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ERN1 Knockout cell line (A549)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

ERN1

Gene ID

2081

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Target region

Knockout region: Exon 2 (based on transcript ERN1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A549			STR Info (Cell bank) Cell Line:A-549
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	12		11	12
VWA	14	14		14	14
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	10	12		10	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ERN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ERN1 gene knockout ERN1 Knockout cell line (A549) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)
More details Order

Catalog#: YC-C016

Size: 1*10^6

Instruction:

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit
More details Order

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
More details Order

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

ERN1 Knockout cell line (Hela)
inquiry More details

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-H731

YKO-H731(Hela)
ERN1 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-HS3206

YKO-HS3206(HCT 116)
ERN1 Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-H896

YKO-H896(HEK293)

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)

Price (USD): -

Catalog#: YC-C016

Size: 1*10^6

Instruction:

inquiry More details

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

Price (USD): -;-;-

Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250

Size: ~50 tests;~250 tests;~250 tests

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

ERN1 Knockout cell line (Hela)

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-H731

inquiry More details
ERN1 Knockout cell line (HCT 116)

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-HS3206

inquiry More details
ERN1 Knockout cell line (HEK293)

Organism: Human

Gene Name: ERN1

Gene ID: 2081

Size: 1*10^6

Catalog#: YKO-H896

inquiry More details

✖

Phone

✖

Contact us✖

* Name：

Institution：

* Project Description：

* How would you like us to contact you?

* E-mail：

Phone：

WhatsAPP：

FaceBook Messenger：

Our Solution Specialists will contact you within 2 business days. Of course, you can also tell us when you would like to be contacted:

Select：

ERN1 Knockout cell line (A549)

Subscribe Us

ERN1 Knockout cell line (A549) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

ERN1 Knockout cell line (Hela) inquiry More details

ERN1 Knockout cell line (HCT 116) inquiry More details

ERN1 Knockout cell line (HEK293) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

KO Cell Lines

ERN1 Knockout cell line (Hela)

ERN1 Knockout cell line (HCT 116)

ERN1 Knockout cell line (HEK293)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human Non-small Cell Lung Cancer Cell Line(A549)
More details Order

High-performance Mouse Genotyping Kit
More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

ERN1 Knockout cell line (Hela)
inquiry More details

ERN1 Knockout cell line (HCT 116)
inquiry More details

ERN1 Knockout cell line (HEK293)
inquiry More details